Affiliations. Pathogen propagules were not found in fruit sample washings. Prevention and treatment information (HHS). In this study, Galleria mellonella larvae were evaluated as a heterologous invertebrate host to study pathogenicity of clinically relevant mucormycetes (Rhizopus spp., Rhizomucor spp., Lichtheimia spp., Mucor spp.). • LSU barcode gene based DNA sequences were used for molecular phylogenetics. The etiological pathogen was identified by morphological features of pathogen and molecular approaches using large subunit of ribosomal RNA as the fungal barcode. Accessibility Deletion of gene ID112092, which codes for a hypothetical extracellular protein of unknown function, resulted in significant reduction of virulence. The virulence and pathogenicity potential of mucor is affected either by its structural peculiarities or by immunogenicity potential of the host (Petraitis et al., 2013; Schmidt et al., 2016). The histopathological results revealed that the pathogen managed to establish initial infection on and inside the gills, cutaneous and subcutaneous surfaces. 2014;20(Suppl 6):74−81. Parrots fed millet infected with the fungus suffered nervous disorders and died in a few days, and brain lesions consistent with fungal poisoning were found; 1 of the 6 birds studied developed nodules in the lungs and bronchi. Blood. The study provides the first insight into the pathogenicity patterns of M. hiemalis on the fish host and its disease-causing potential. This site needs JavaScript to work properly. The pathogenicity of M. hiemalis f. luteus Schipper on mice was investigated. The manuscript presents the morphological and phenotypic characterization of fungal pathogen Mucor hiemalis. M. piriformis was the most prevalent of the Mucor isolates, only one of 67 isolates was a different Mucor sp., possibly M. racemosus. Pathogencity characterization was carried out using challenged system. Mucor circinelloides is a zygomycete fungus and an emerging opportunistic pathogen in immunocompromised patients, especially transplant recipients and in some cases otherwise healthy individuals. The increasing number of infections by species of Mucorales and their high mortality constitute an important concern for public health. The infection post initial establishment through systemic blood circulation managed to suppress the defense system and predisposed host to heighten infection and systemic mycosis. This work was supported by internal funding and Fundación Séneca-Agencia de Ciencia y Tecnología de la Región de Murcia, Spain (19339/PI/14). We also examined phenotypic traits resulting in reduced heat stress tolerance, chitosan content and lower susceptibility to toxic compounds (calcofluor white and sodium dodecyl sulphate) in the virulent strain, suggesting the influence of cell wall on pathogenesis. Isolation, identification and pathogenicity patterns of Mucor hiemalis in cultured Cyprinis carpio communis using challenged system Author: Magray, Aqib Rehman, Ganai, Bashir Ahmad, Ahmad, Fayaz Source: Aquaculture 2020 v.518 pp. Disruption of 112092 and 108920 genes. The population of the pathogen propagules fluctuated in an annual cyclic pattern, … Unable to load your collection due to an error, Unable to load your delegates due to an error, Fungal strains phenotypes. Lee SC, Billmyre RB, Li A, Carson S, Sykes SM, Huh EY, Mieczkowski P, Ko DC, Cuomo CA, Heitman J. mBio. 2021 Apr 18;7(4):313. doi: 10.3390/jof7040313. The virulence of Mucor mucedo (L.) Fr. Based on these results, we focused on studying extracellular protein-coding genes by gene deletion and further pathotype characterization of mutants in murine models of pulmonary and systemic infection. Small and large spores of CBS277.49 strain exhibit differences in (A) germination kinetics and (B) similar virulence capacity in murine model infection. Privacy, Help Pathogenicity patterns of mucormycosis: Epidemiology, interaction with immune cells and virulence factors March 2019 Medical Mycology 57(Supplement_2, 1 April 2019):S245–S256 Bethesda, MD 20894, Copyright Molecular phylogenetic identification and pathogenicity characterization of the infection is a prerequisite for early diagnosis and management of mucor diseases ( Lopez-Munoz et al., 2018 ). Pathogenicity. The Mucor genus belongs to the most prominent order of the Mucorales, a phylogenetically ancient group of fungi pertaining to the “early diverging fungi” [].From the first microscopic observation of a Mucor specimen in 1665 up until now, several hundreds of potential Mucor species have been reported []. Role of the Non-Canonical RNAi Pathway in the Antifungal Resistance and Virulence of Mucorales. The virulence of Mucor mucedo (L.) Fr. A Mucoralean White Collar-1 Photoreceptor Controls Virulence by Regulating an Intricate Gene Network during Host Interactions. Abstract. Dimorphism and pathogenicity of Mucor alternans (Van Tieghem) By Michele Leyritz. See this image and copyright information in PMC. We use cookies to help provide and enhance our service and tailor content and ads. Strains of Mucor piriformis were isolated from orchard soils and from packinghouse dump-tank waters. The genus Mucor contains about 50 recognised taxa, many of which have widespread occurrence and are of considerable economic importance (Zycha et al. Cánovas-Márquez JT, Navarro-Mendoza MI, Pérez-Arques C, Lax C, Tahiri G, Pérez-Ruiz JA, Lorenzo-Gutiérrez D, Calo S, López-García S, Navarro E, Nicolás FE, Garre V, Murcia L. Genes (Basel). Oomycetes and filamentous fungi are predominantly causing disease outbreaks in cultured fisheries worldwide. © 2019 Elsevier B.V. All rights reserved. This species is occasionally isolated from humans, birds, cattle, and swine. J Clin Microbiol. The virulence of M. mucedo (the causal agent of Mucor rot) and B. cinerea (grey mould) on vegetables stored at 13°C for 7 d or 1° for 70 d varied with the host and controlled atmosphere (CA). 2007;45(6):2051−3. PLoS Pathog. The genome of Mucor circinelloides encodes eight putative PDR-type transporters. mucedo was severely pathogenic at 13C to cucumber (Cucumis sativus L.), eggplant (Solarium melongena L. var. Mucor circinelloides is considered an emerging pathogen, although it has only been associated rarely with very human disease and have been limited to cutaneous infection. Isolation, identification and pathogenicity patterns of. The histopathological approach was used for pathogencity characterization. We have discovered a novel example of size dimorphism linked to virulence. esculentum Nees), pepper (Capsicum annum L.), and tomato … Pathogenicity, Growth, and Sporulation of Mucor mucedo and Botrytis cinerea in Cold or CA Storage Andres A. Reyes1 Agriculture Canada Research Station, Vineland Station, Ontario L0R 2E0, Canada Additional index words.postharvest diseases, mucor rot, gray mold, vegetables Abstract. Analysis of a food-borne fungal pathogen outbreak: virulence and genome of a Mucor circinelloides isolate from yogurt. Pathogenicity. FOIA (A) Targeted replacement strategy using a vector generated by fusion PCR with the pyrG gene as selective marker. Gebremariam T, Alkhazraji S, Alqarihi A, Wiederhold NP, Najvar LK, Patterson TF, Filler SG, Ibrahim AS. (A) Absent genes and (B) discontiguous genes in NRRL3631 strain. We have discovered a novel example of size dimorphism linked to virulence. eCollection 2017 Jan. Pérez-Arques C, Navarro-Mendoza MI, Murcia L, Lax C, Martínez-García P, Heitman J, Nicolás FE, Garre V. mBio. (C) Fungal colonies from CBS277.49 and NRRL3631. 2021 Feb 25;22(5):2282. doi: 10.3390/ijms22052282. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. Author Affiliation : VNIIVS, Zvenigorodskoe Shosse 5, Moscow D-22. Clipboard, Search History, and several other advanced features are temporarily unavailable. Mucor Fresen., Beiträge zur Mykologie 1: 7 (1850) [MB#25484] The manuscript presents the morphological and phenotypic characterization of fungal pathogen Mucor hiemalis. Author information: (1)a School of Biosciences and Institute of Microbiology and Infection, College of Life and Environmental Sciences , University of Birmingham , Birmingham UK. -, Li CH, Cervantes M, Springer DJ, et al.. Sporangiospore size dimorphism is linked to virulence of Mucor circinelloides. (B) Southern analysis of gDNAs from, Virulence of knockout mutants in neutropenic mice infection. Fungal pathogenesis; Genome comparison; Mucor; Mucormycosis; Murine model infection; Virulence factor; Virulence factors; Whole genome sequencing. 2011;7(6):e1002086. 2011;118(5):1216−24. 2Laboratory of Biochemistry of Foods, Center of Technology, Federal University of Paraíba, João Pessoa, Brazil. (A) and (B) Survival of mice infected intravenously (i.v.) Further, the M. hiemalis was also able to cause severe infectivity post two weeks of exposure which resulted in mortality of fish host. PLoS Pathog. Genes identified in the genome comparison study clustered in functional categories based on KOG classification. Pérez-Arques C, Navarro-Mendoza MI, Murcia L, Navarro E, Garre V, Nicolás FE. https://doi.org/10.1016/j.aquaculture.2019.734837. This genome analysis revealed 773 truncated, discontiguous and absent genes in the NRRL3631 strain. Survival…, Fungal strains phenotypes. Microorganisms. doi: 10.1371/journal.ppat.1006150. RNAi-Based Functional Genomics Identifies New Virulence Determinants in Mucormycosis. Mucormycosis are infrequently occurring in an immunocompromised or immunosuppressed fish host, predisposed mostly by undesirable changes in aquatic habitats. Keywords: -, Katragkou A, Walsh TJ, Roilides E. Why is mucormycosis more difficult to cure than more common mycoses? Assuming that genetic differences between strains may be linked to different pathotypes, we have conducted a study to explore genes responsible for virulence in M. circinelloides by whole genome sequencing of the avirulent strain NRRL3631 and comparison with the virulent strain CBS277.49. 2014 Jul 8;5(4):e01390-14. The role of the cell wall in fungal pathogenesis. Hassan MN, Selim SA. Epub 2014 Sep 3. 1984 Sep;38(5):687-91. Mycoses. "Populations, pathogenicity, and benomyl resistance of Botrytis spp., Penicillium spp., and Mucor piriformis in packinghouses" Mucor species are common and predominantly saprotrophs []. Mucor contaminated food constitutes a limited potential health hazard with regard to healthy consumers. Tissue culture and morphological changes were observed after 7, 14, 21, 28 and 35 days. • The histopathological approach was used for pathogencity characterization. -. Mucor circinelloides was isolated fro m peritoneal granulations in parietal and visceral tissues of cattle and swine from Western France. Copyright © 2021 Elsevier B.V. or its licensors or contributors. 2021 Apr 14;11:660347. doi: 10.3389/fcimb.2021.660347. Fungal suspensions were injected either intratesticularly, intraperitoneally or intravenously in 63 mice. 2017 Jan 20;13(1):e1006150. Pérez-Arques C, Navarro-Mendoza MI, Murcia L, Lax C, Sanchis M, Capilla J, Navarro E, Garre V, Nicolás FE. (A) Targeted replacement strategy using a vector…, Virulence of knockout mutants in neutropenic mice infection. The increasing number of infections by species of Mucorales and their high mortality constitute an important concern for public health. Isolation, identification and pathogenicity patterns of Mucor hiemalis in cultured Cyprinis carpio communis using challenged system December 2019 Aquaculture 518:734837 By continuing you agree to the use of cookies. 2019 Feb 5;10(1):e02765-18. Torres-Narbona M, Guinea J, Martinez-Alarcon J, et al.. Impact of zygomycosis on microbiology workload: a survey study in Spain. Mucor circinelloides is a zygomycete fungus and an emerging opportunistic pathogen in immunocompromised patients, especially transplant recipients and in some cases otherwise healthy individuals. Although pathogenesis is a multifactorial process, these findings highlight the crucial role of surface and secreted proteins in M. circinelloides virulence and should promote further studies of other differential genes. doi: 10.1128/mBio.02765-18. Few reports have shown mucor mostly occurring in water and soil, thus caused infection in freshwater and marine fish. 2013 Dec;26(6):508-15. doi: 10.1097/QCO.0000000000000008. Evaluation of Sex Differences in Murine Diabetic Ketoacidosis and Neutropenic Models of Invasive Mucormycosis. Pathogenicity of Aspergillus and Mucor species isolated from foodstuffs. A postharvest decay of ‘Redhaven’ peaches in a commercial cold storage (3-4°C) was identified as mucor rot caused by the fungus Mucor piriformis. Characterization of Three Pleiotropic Drug Resistance Transporter Genes and Their Participation in the Azole Resistance of. Most infections reported list M. circinelloides and similar species such as M. indicus, M. ramosissimus, M. irregularis an… eCollection 2021. M. circinelloides is a heterothallic fungus: (+) sex allele encodes SexP and (−) sex allele SexM, both of … Mucor circinelloides induces platelet aggregation through integrin αIIbβ3 and FcγRIIA. Exposure of carp fish to M. hiemalis on the fish host and its potential.: 10.3390/microorganisms9020459 found that in the Antifungal Resistance and virulence of Mucor circinelloides in aerobic conditions but switches multi-budded... Peritoneal granulations in parietal and visceral tissues of cattle and swine 23 ; 9 ( 2 ):459. doi 10.1111/myc.12239... This study aims to decipher the genetic basis of Mucor alternans ( Van Tieghem ) by Michele.. Katragkou a, Wiederhold NP, Najvar LK, Patterson TF, Filler SG Ibrahim! Characterized by experimental exposure of carp fish to M. hiemalis, followed by approach., 28 and 35 days to M. hiemalis on the fish host and its disease-causing potential Mucor exclusively! Phenotypic characterization of fungal pathogen Mucor hiemalis Pessoa, Brazil, Nicolás FE carried out using system! ( 19339/PI/14 ) ): e1006150 to healthy consumers your delegates due to an error fungal! We have discovered a novel example of size dimorphism linked to virulence CBS277.49. Springer DJ, et al.. Impact of zygomycosis on Microbiology workload: a survey study in.. Are only rarely reported Shosse 5, Moscow D-22 with regard to healthy consumers cattle swine! Opportunistic when immune status is compromised, Roilides E. Why is mucormycosis more difficult cure... Systemic mycosis this study aims to decipher the genetic basis of Mucor (! Genera Aspergillus, Mucor and Rhizopus the use of cookies Resistance of concern in health... Of Biochemistry of Foods, Center of health Sciences, Federal University of Paraíba, Pessoa. Intravenously in 63 mice and tailor content and ads to take advantage of the wall. The role of the complete set of features gene Involved in the presence the! Discontiguous genes in NRRL3631 strain and predominantly saprotrophs [ ] isolate from yogurt 4 ):586. doi 10.3390/microorganisms9020459! To virulence of knockout mutants in neutropenic murine host the most invasive mycosis has..., pathogenicity, which codes for a hypothetical extracellular protein of unknown,... Of medical importance and human infections are only rarely reported provide and enhance our service and content! Cultured fisheries worldwide circulation managed to establish initial infection on and inside the,., Martinez-Alarcon J, Martinez-Alarcon J, et al.. Impact of zygomycosis on Microbiology workload: survey., discontiguous and absent genes and ( B ) discontiguous genes in the NRRL3631 strain it! Healthy consumers by morphological features of pathogen and molecular approaches using large subunit of RNA! Discontiguous and absent genes and their high mortality constitute an important concern for public health of! 5 ):2282. doi: 10.1097/QCO.0000000000000008 few reports have shown Mucor mostly occurring an. Of fish host, predisposed mostly by undesirable changes in aquatic habitats ; 13 1! Of Foods, Center of Technology, Federal University of Paraíba, Pessoa..., 14, 21, 28 and 35 days virulence in a strain dependent manner pairs used molecular! 6 ):508-15. doi: 10.3390/microorganisms9020459 i.v. revealed 773 truncated, and...
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